In a statement, her family confirmed she died at her property in Neergabby in the shire of Gingin, about 80 kilometres north of Perth.
“It is with utterly broken hearts that we announce that Virginia passed away [on Friday night] at her farm in Western Australia,” the statement said.
“She lost her life to suicide, after being a lifelong victim of sexual abuse and sex trafficking.
“Virginia was a fierce warrior in the fight against sexual abuse and sex trafficking.
“She was the light that lifted so many survivors. Despite all the adversity she faced in her life, she shone so bright.
She will be missed beyond measure.”
A WA Police spokesperson said emergency services received a report that a woman had been located unresponsive at a residence in Neergabby just before 10pm on Friday.
“Police and St John WA attended and provided emergency first aid. Sadly, the 41-year-old woman was declared deceased at the scene,” the spokesperson said.
“The death is being investigated by Major Crime detectives; early indication is the death is not suspicious.
Her family said her children Christian, Noah, and Emily were the light of her life.
“It was when she held her newborn daughter in her arms that Virginia realised she had to fight back against those who had abused her and so many others,” they said.
“There are no words that can express the grave loss we feel today with the passing of our sweet Virginia.
“She was heroic and will always be remembered for her incredible courage and loving spirit.
“In the end, the toll of abuse is so heavy that it became unbearable for Virginia to handle its weight.”
Ms Giuffre was a prominent accuser of the late financier Jeffrey Epstein and Prince Andrew, the Duke of York.
She alleged she was trafficked for sex to the Duke of York by Epstein on several occasions when she was a teenager.The duke has repeatedly denied those allegations.
A settlement was reached in February 2022 in a civil case brought by Ms Giuffre against Prince Andrew.
The deal avoided a trial that would have brought further embarrassment to the monarchy.
Ms Giuffre asserted that she met the duke while she travelled frequently with Epstein between 2000 and 2002.
Her lawyers maintain she was “on call for Epstein for sexual purposes” and was “lent out to other powerful men,” including the prince.
Prince Andrew has repeatedly denied Ms Guiffre’s accusations that he forced her to have sex more than two decades ago at the London home of Ghislaine Maxwell, Epstein’s associate, and allegations he abused her at Epstein’s properties in Manhattan and the British Virgin Islands.
The settlement came just over a month after a US judge rejected Prince Andrew’s attempt to win an early dismissal of the lawsuit, meaning depositions and other evidence-gathering could commence.
After the ruling, the duke — who had already stepped back from royal duties — was stripped of his honorary military titles and roles and leadership of various charities, known as royal patronages.
He also can no longer use the title “his royal highness″ in official settings.In late March, Ms Giuffre posted on Instagram saying she had been involved in a bus crash and had “four days to live” after doctors had told her she was suffering kidney failure.
Days later, her family said Ms Giuffre was in a “serious condition while receiving medical care” after a crash.
However, the Perth hospital where she was staying denied that characterisation, with police maintaining the crash was “minor”.